Fluorescent microscopy: nta kev tshawb fawb

Lub sij hawm "tshuab kuaj kab mob" muaj Greek cov hauv paus hniav. Nyob rau hauv translation nws txhais tau hais tias txoj kev tshawb no ntawm cov khoom uas high-precision seev. . Nyob rau hauv xyoo tsis ntev los ua nce nrov fluorescent thiab electron microscopy.

kev daws teeb meem

Nyob rau hauv nws, raws li ib tug txoj cai, to taub qhov tsawg kawg nkaus deb ntawm uas tej zaum yuav kom meej meej distinguishable khoom. Nyob rau hauv kev daws teeb meem muaj peev xwm ntawm cov khoom degree ntawm allergic yuav nyob ntawm seb nyob rau hauv lub me me ntiaj teb no, yuav pom tau qhov loj ntawm ib tug caij. Nrog ib tug loj nce nyob rau hauv ciam teb chaw yuav tshuam. Raws li, muaj qee yam kev txwv, tshaj uas tus mus kom ze ntawm lub ntsiab yog meaningless.

Cov kev fluorescence microscopy: mechanism

Thaum lub zog hloov dua siab tshiab uas absorbs tshuaj mus rau hauv pom tawg tshwm sim ci ntsa iab. Nws yog hu ua luminescence. Qhov no tshwm sim yog vim lub fact tias ib txhia tshuaj nyob rau hauv tus ntawm lub teeb pib emit rays los ntawm lwm yam (feem ntau yog cov loj loj) wavelength. Tsis tas li ntawd, ib co khoom uas muaj nyob rau hauv ib txwm tej yam kev mob teeb pom kev zoo teev xim nyob rau hauv tus ntawm ultraviolet lub teeb hloov nws cov xim.

tseem

Ib yam khoom uas tsis tau pom nyob rau hauv UV teeb, yuav emit kaj ci, yog hais tias nws yog kho nrog ib tug tshwj xeeb tshuaj. Nyob rau hauv nws cov ntsiab ntawm cov xim sib txawv ntawm lub teeb nyob rau hauv qhov tsaus ntuj. radiant siv yog sib txawv, tab sis raws li ib tug txoj cai, nws yog me me. Nyob rau hauv no hais txog, fluorescence microscopy yog zoo nyob rau hauv ib tug darkened chav tsev. Thaum uas siv cov no hom ntawm kev tshawb fawb yuav pom nyob rau hauv lub teeb ntawm tus kwv, uas nws radiates. Cov tshuaj nyob tus yeees ntawm ntaub so ntswg, lub hlwb yuav muaj kev cuam tshuam zoo ntawm cov kev tshawb no. Fluorescence microscopy yog suav tias yog mus rau ib tug tej yam raws li histochemical txoj kev tshawb no.

kev faib

может быть первичной или вторичной. Fluorescence microscopy yuav ua tau thawj los sis theem nrab. Nyob rau hauv lub caij nyoog kawg no cov ntaub ntawv, tus kwv yog ua los ntawm tshwj xeeb tebchaw muab luminescence. основывается на собственной способности исследуемого элемента излучать свет. Primary fluorescent microscopy raws li nyob rau hauv lawv tus kheej muaj peev xwm ntawm ib tug caij mus emit teeb.

cov khoom

Fluorescence microscopy yog tau siv ib tug ntau yam ntawm pab kiag li lawm. Lub illuminator ua hauj lwm pab raws li cov tub ntxhais ntawm lub caij. Nws yog txawm peem rau nrog UV teeb. Nyob rau hauv tas li ntawd, tus ntaus ntawv siv ib tug txheej ntawm cov ntxaij lim dej. Nyob rau hauv ib co seev yog ib tug heev ob peb txawv configurations. Nyob ntawm seb uas tsuas yog siv rau luminescence excitation - ultraviolet los yog xiav, nruab nrab ntawm lub teeb qhov chaw thiab tus kwv nyob rau hauv kev tshawb nrhiav yog muab tso rau ib tug tsim nyog lim. Txij li thaum luminescence me caij energetically weaker excitation lub teeb, nws yuav tsum ntes tau xwb nyob rau ib yam kev mob. Seem ntawm rays txuas los ntawm qhov twg los yuav tsum tau txiav tawm daj-ntsuab lim. Nws yog muab tso rau lub twj paj nruag eyepiece. Qhov tseem pronounced nyhuv luminescence yuav tshwm sim thaum lub lim yog kiag li txiav tawm lub rays ntawm lub teeb ntsaws los ntawm lub hauv paus.

Yuav ua li cas yog lub installation nyob rau hauv pom lub teeb? Nyob rau hauv nws muaj yog ib qho ci qhov twg los thiab ib lom tshuab kuaj kab mob. Nruab nrab ntawm lub iav chav tsev thiab ib lub teeb mounted xiav-violet lim. Qhov no yuav ua tau FS-1, UFS-3 thiab thiaj li nyob. Daj lim tabtom haum tus eyepiece ntawm lub tshuab kuaj kab. Nrog lawv pab, muaj ib tug kwv ntog xiav-violet lub teeb. Nws zoo siab heev luminescence. Tab sis qhov no teeb muaj peev xwm cuam tshuam nrog cov zaub illumination. Yog li ntawd, nyob rau hauv txoj kev mus rau lub qhov muag nws clipped ib tug daj lim. Installation ntawm teeb pom kev zoo los ntawm Koehler txoj kev, nrog ib tug tsuas. Diaphragm condenser yuav tsum siab qhib. Nyob rau hauv txoj kev tshawb no nws yog ib qho tseem ceeb rau siv ib tug uas tsis yog-fluorescent immersion roj. Yuav kom txo tau cov intrinsic emission ntxiv thereto nitrobenzene (2-10 dauv / 1 g).

Fluorescent microscopy: daim ntaub ntawv nyob rau hauv microbiology

Qhov zoo ntawm no hom ntawm kev tshawb fawb yog:

1. Cov tseem tau xim dluab.
2. High zoo self-illuminating ntsiab rau ib tug dub keeb kwm yav dhau.
3. Kom paub tias thiab localization ntawm tej hom ntawm cov kab mob thiab cov kab mob.
4. Cov tau kawm pob tshab thiab opaque nyob kab mob.
5. Txoj kev tshawb no ntawm lub neej dab nyob rau hauv lawv puab.

Nws tseem yog tsim nyog hais tias lub fluorescent microscopy txhawb lub finest txoj kev histology thiab cytochemistry, ceev ceev mob.